Systematic Mapping of Bacterial CRISPRa Design Rules and Implications for Synergistic Gene Activation
Cholpisit Kiattisewee, Ava V. Karanjia, Ryan A. L. Cardiff, Kira E. Olander, Pansa Leejareon, Sarah Alvi, James M. Carothers, Jesse G. Zalatan
This study systematically maps bacterial CRISPRa design rules and finds no synergistic activation, highlighting mechanistic differences from eukaryotic systems.
ACS Synthetic Biology · Jul 22, 2025Peer-reviewedSynthetic Biology