Cholpisit Kiattisewee

Conjugative serine recombinase engineering enables genome integration in transformation-resistant bacteria and bioproduction pathway prototyping

This paper introduces cSAGE, a conjugation-based genome engineering platform that enables site-specific integration in transformation-resistant bacteria, demonstrating its utility in bioproduction pathway prototyping.

CRISPRi-assisted E. coli strains increase success rate of burdensome construct cloning

The paper introduces CRISPRi-assisted E. coli strains to improve cloning success for burdensome genetic constructs by reducing metabolic burden during cloning.

Systematic Mapping of Bacterial CRISPRa Design Rules and Implications for Synergistic Gene Activation

This study systematically maps bacterial CRISPRa design rules and finds no synergistic activation, highlighting mechanistic differences from eukaryotic systems.

Guide RNA structure design enables combinatorial CRISPRa programs for biosynthetic profiling

The paper designs guide RNA structures for CRISPRa to optimize multi-gene expression in bacterial metabolic pathways, achieving higher product titers and identifying pathway bottlenecks.

Expanding the scope of bacterial CRISPR activation with PAM-flexible dCas9 variants

This study evaluates PAM-flexible dCas9 variants for bacterial CRISPR activation and repression, finding trade-offs between PAM flexibility and repression efficiency.

Effective CRISPRa-Mediated Control of Gene Expression in Bacteria Must Overcome Strict Target Site Requirements

This study identifies strict target site requirements for effective CRISPRa in bacteria and demonstrates a dCas9 variant with expanded PAM specificity to overcome these limitations.