Ryan A. L. Cardiff

This paper introduces cSAGE, a conjugation-based genome engineering platform that enables site-specific integration in transformation-resistant bacteria, demonstrating its utility in bioproduction pathway prototyping.

This study systematically maps bacterial CRISPRa design rules and finds no synergistic activation, highlighting mechanistic differences from eukaryotic systems.

This paper engineers a carbon-conserving cell-free system in E. coli to produce malate from formate and bicarbonate, achieving a 15-fold increase in titer and reducing background reactions by 6-fold.

The paper designs guide RNA structures for CRISPRa to optimize multi-gene expression in bacterial metabolic pathways, achieving higher product titers and identifying pathway bottlenecks.

The paper develops CRISPR-Cas tools to independently control transcription and translation in E. coli, showing reduced polar effects with dCas13 and enabling multiplexed gene regulation for biosynthesis.