Discovery of excited state proton transfer in flavin-based fluorescent protein with large Stokes shift
Andrey Nikolaev, Elena Markeeva, Eugene G. Maksimov, Anna Yudenko, Elena V. Tropina, Kirill N. Boldyrev, Yuliya Nikolaeva, Dmitry A. Cherepanov, Kirill Kovalev, Yuqi Yang, Valentin I. Borshchevskiy, Elizaveta Kuznetsova, Alexander Kuzmin, Oleg Semenov, Alina Remeeva, Ivan Gushchin
The paper discovers a flavin-based fluorescent protein, LSSFbFP, with a large Stokes shift, achieved through excited state proton transfer, and validates its mechanism using spectroscopy and crystallography.
bioRxiv · Jun 7, 2025 · PreprintBiochemistry