Kirill Kovalev

Structural Basis for Near Infrared Absorption and Noncanonical Photoisomerization in Neorhodopsin

This study investigates the structural basis of near-infrared absorption and noncanonical photoisomerization in neorhodopsin using crystallographic, spectroscopic, and theoretical methods.

EasyGrid: A versatile platform for automated cryo-EM sample preparation and quality control

EasyGrid automates cryo-EM sample preparation and quality control, improving vitrification for macromolecules and mammalian cells.

Microsecond Time-Resolved Cryo-EM Based on Jet Vitrification

This paper introduces a method to achieve microsecond time resolution in cryo-EM using jet vitrification, enabling observation of protein dynamics with near-atomic resolution.

Structural basis for no retinal binding in flotillin-associated rhodopsins

The paper presents cryo-EM structures of FArhodopsin and proteorhodopsin, showing no retinal binding despite conserved lysine and similar fold to retinal-binding rhodopsins, with mutations identified that prevent retinal binding.

Discovery of excited state proton transfer in flavin-based fluorescent protein with large Stokes shift

The paper discovers a flavin-based fluorescent protein, LSSFbFP, with a large Stokes shift, achieved through excited state proton transfer, and validates its mechanism using spectroscopy and crystallography.