Huibao Feng

This study uses CRISPRi screening to identify transcription factors crucial for growth and carbon utilization in Clostridium ljungdahlii, improving ethanol production through deoR optimization.

This paper introduces dSort-Seq, a deep-learning-assisted Sort-Seq method for high-throughput profiling of gene expression characteristics with high precision, and demonstrates its application in studying noise and dose-response relationships in E. coli.

The paper measures thermodynamic parameters of RNA/DNA hybrid duplexes and develops a nearest-neighbor parameter set with high accuracy for predicting their properties.

GLiDe is a web-based tool for designing CRISPRi sgRNA libraries in prokaryotes, improving off-target detection and providing a database for 1,397 species.