Single-molecule live-cell RNA imaging with CRISPR-Csm
Chenglong Xia, David Colognori, X. Jiang, Ke Xu, Jennifer A. Doudna
This paper introduces smLiveFISH, a method for single-molecule live-cell imaging of endogenous RNA using CRISPR-Csm, and identifies two distinct mRNA localization mechanisms.
Nature Biotechnology · Feb 18, 2025Peer-reviewedBiophysics