Ke Xu

The paper introduces a method for live-cell super-resolution microscopy using fluorogenic HaloTag ligands and BF dyes, enabling single-molecule localization and diffusivity mapping of proteins.

This study shows that Myosin1E adapts to increased membrane tension by recruiting to more clathrin-mediated endocytosis sites to enhance actin network formation and promote internalization.

SpeedyTrack enables microsecond wide-field single-molecule tracking and super-resolution mapping using EM-CCD vertical shift without modifying existing microscopy setups.

ExSPM reveals spatiotemporal heterogeneities in lipid droplet polarity, showing dynamics and pathway-specific kinetics of lipid metabolism.

This study introduces pc-SMdM, a super-resolution technique to map nanoscale diffusivity on cellular membranes, revealing clusters influenced by cholesterol and membrane structure.

This paper introduces smLiveFISH, a method for single-molecule live-cell imaging of endogenous RNA using CRISPR-Csm, and identifies two distinct mRNA localization mechanisms.

The paper quantifies single-molecule diffusivity in Xenopus egg extracts to reveal the cytoplasm's physicochemical properties, showing charge-dependent diffusion and the role of RNA and actin.

This study shows that vimentin cytoskeleton assembly is sensitive to net charge states and Coulomb repulsion, with hypotonic stress causing rapid disassembly due to reduced charge screening.

The paper performs genome-wide CRISPRi/a screens in human neurons to identify gene functions related to oxidative stress and neurodegenerative diseases, finding that lysosomal failure links to ferroptosis.