Pharmacology & ToxicologybioRxivPreprint
Essential strategies for the detection of constitutive and ligand-dependent Gi-directed activity of 7TM receptors using bioluminescence resonance energy transfer
Sofia Endzhievskaya, Kirti Kandhwal Chahal, JULIE A. RESNICK…·Irina Kufareva
This study develops and validates BRET-based assays to detect constitutive and ligand-dependent Gi-directed activity in GiPCRs, revealing novel regulatory mechanisms of Smoothened.
DOI 10.1101/2024.12.04.626681