Molecular BiologyPeer-reviewed journalPeer-reviewed
Comparison of the 2-CT method and the 2-ΔΔCT method for real-time qPCR data analysis
Lixiang Feng, Rongqian Zhao, Kui Zhang, Wenxing Yang
This paper compares two RT-qPCR analysis methods, showing the 2-ΔΔCT method has systematic bias and the 2-CT method provides more accurate normalization.
DOI 10.12182/20250960402