Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology
Noor Radde, Genevieve A. Mortensen, Diya Bhat, Shireen Shah, Joseph J. Clements, Sean P. Leonard, Matthew J. McGuffie, Dennis M. Mishler, Jeffrey E. Barrick
The paper measures the growth burden of 301 BioBricks in E. coli and finds that 19.6% reduce growth, with most burdens explained by gene expression resource diversion.
Nature Communications · Jul 24, 2024Peer-reviewedSynthetic Biology