Dennis M. Mishler

Caffeinated Coli: Inspiring the Next Generation of Scientists through Synthetic Biology

This paper describes an educational module using genetically engineered E. coli to teach high school students about synthetic biology and caffeine measurement through hands-on experiments.

Refactored genetic parts for modular assembly of the E. coli MccV type I secretion system used to screen class II microcin candidates from plant-associated bacteria

The paper refactors the E. coli MccV type I secretion system into modular genetic parts for secreting small proteins and screens class II microcins from plant-associated bacteria.

Engineered Acinetobacter baylyi ADP1-ISx cells are sensitive DNA biosensors for antibiotic resistance genes and a fungal pathogen of bats

The paper describes engineered Acinetobacter baylyi biosensors that detect specific DNA sequences with high sensitivity.

Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology

The paper measures the growth burden of 301 BioBricks in E. coli and finds that 19.6% reduce growth, with most burdens explained by gene expression resource diversion.

Robust Estimation of Bacterial Cell Count from Optical Density

The paper evaluates three OD calibration protocols for bacterial cell count estimation and recommends serial dilution of silica microspheres for its precision and compatibility with flow cytometry.