Sean P. Leonard

The paper shows that unobserved protein sequence space contains many functional proteins, challenging the assumption that existing proteins cover most functional space.

This paper introduces a method to create overlapping genes by inserting an inner gene into an outer gene, demonstrating functionality and applications in synthetic biology.

The paper measures the growth burden of 301 BioBricks in E. coli and finds that 19.6% reduce growth, with most burdens explained by gene expression resource diversion.

GENTANGLE is a computational tool for designing overlapping gene pairs in microbial genomes, enabling safer genetic engineering through gene entanglements.

This paper demonstrates how sequence entanglement and adaptive evolution can stabilize synthetic genetic circuits by altering mutational landscapes and reducing circuit burden.

This paper describes a method to study gene function in bees using engineered gut bacteria to induce RNA interference, achieving up to 75% gene knockdown.

The paper improves genome engineering methods for Acinetobacter baylyi ADP1, achieving genome streamlining through targeted deletions and CRISPR-based validation.