Synthetic BiologyNucleic Acids ResearchPeer-reviewed
In Vivo Pathway Optimization in Yeast via LoxPsym-Mediated Shuffling of Upstream Activating Sequences
Ciaran Ruehmkorff, Ali Tafazoli Yazdi, Lena Hochrein
The paper introduces PULSE, a method for in vivo promoter engineering in yeast to optimize biosynthetic pathways, achieving an eightfold increase in β-carotene production and improved growth on high xylose concentrations.
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