GenomicsbioRxivPreprint
The Illumina Stranded mRNA protocol is not strongly stranded for mRNA with low U content
Olga Menshikova, Isabelle Nuez, Virginie Courtier‐Orgogozo
The paper shows that the Illumina Stranded mRNA protocol can generate sense reads for low U-content transcripts, affecting gene expression estimates.
DOI 10.64898/2026.07.09.737636