Author

Yu Shirai

Harvard UniversityORCID 0009-0009-0346-7873Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Organism:Arabidopsis· 1 Research Focus:cell type annotation· 1 Research Focus:gene regulatory network inference· 1 Approach:genetic perturbation / CRISPR· 1 Research Focus:genome assembly· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Ashley Watts· 1 shared Cassandra G. M. ExtavouriD· 1 shared Hirotaka KanukaiD· 1 shared Jonchee A. KaoiD· 1 shared Manabu OtéiD· 1 shared Momoyo Takahashi· 1 shared Takaaki DaimoniD· 1 shared Yousuf HashmiiD· 1 shared

Activity by Year

2023-2026 · peak 1/yr

Co-Author Collaboration Network

8 top collaborators · 12 mutual co-author links

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1 shared paperDevelopmental Biology

Ashley Watts

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2 papers

Recent·Most cited

Developmental BiologybioRxivPreprintJul 31, 2026

A nuclear targeting approach enables efficient transgenesis in the milkweed bug Oncopeltus fasciatus

Yu Shirai, Yousuf Hashmi, Ashley Watts…·Cassandra G. M. Extavour

This paper develops an efficient transgenesis system in the milkweed bug Oncopeltus fasciatus using nuclear targeting and piggyBac, enabling genetic manipulations and expanding its use as a model organism.

1 citation

experimental assaygenetic perturbation / CRISPR

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GeneticsbioRxivPreprintApr 7, 2023

DIPA-CRISPR gene editing in the yellow fever mosquito Aedes aegypti (Diptera: Culicidae)

Yu Shirai, Momoyo Takahashi, Manabu Oté…·Takaaki Daimon

This study applies DIPA-CRISPR to Aedes aegypti, demonstrating its effectiveness in polytrophic meroistic ovaries and confirming optimal injection timing for gene editing.

DOI 10.1101/2023.04.07.535996

Arabidopsisexperimental assay

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