Sofía Fajardo‐Callejón

This paper demonstrates a method to enrich lysosomes from low cell numbers by combining plasma membrane destabilization with immunoprecipitation, improving proteome characterization from half a million cells.

The paper identifies that lysosomal cholesterol exchange regulates motility through mTOR-dependent LAMTOR1 phosphorylation, revealing a feedback mechanism affecting mTORC1 activity and lysosomal movement.

The paper develops a mass spectrometry-based method to absolutely quantify lysosomal proteins in mouse cells, revealing expression dynamics and subcellular distribution differences.