BiochemistryeLifePeer-reviewed
Observing one-divalent-metal-ion dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo
Caleb Chang, Grace Zhou, Yang Gao
This study uses time-resolved crystallography to investigate the catalytic mechanism of His-Me family I-PpoI nuclease, revealing the role of a single divalent metal ion and histidine in DNA hydrolysis.