Developmentally regulated actin-microtubule crosstalk in Drosophila oogenesis

Abstract

Actin-microtubules (MT) interactions are essential, but how those mechanisms are orchestrated in complex developing systems is poorly understood. Here we show that actin-MT crosstalk regulates actin cable assembly and the assembly and organization of MTs in Drosophila nurse cells during oogenesis. We found that a stable MT meshwork develops concurrently with actin cable initiation and requires acetylation for its maintenance. These {gamma}-tubulin nucleated MTs are cortically tethered via Patronin and Shortstop, extend into the cytoplasm, and coalign with the elongating actin cables. The MT network promotes the cortical enrichment of the actin assembly factors Diaphanous and Enabled and loss of MTs dramatically reduced actin cable density and growth rate. We further demonstrated that actin filament assembly promotes cortical tethering of MTs and that loss of the actin filament bundler Villin/Quail results in fewer, shorter, and more highly coaligned MTs. Together, our data reveal multiple modes of coordinated actin-MT crosstalk producing actin cable-MT network synergy that is instrumental for oogenesis. SummaryThis study demonstrates actin-microtubule crosstalk in Drosophila oogenesis, revealing coregulation between actin filament assembly/bundling and microtubule nucleation/organization, highlighting the coordinated regulation of the cytoskeleton required during development.