bioRxivCell BiologyPreprint
GOA-1 regulates spermathecal transits
Fereshteh SadeghianiD, Virginie Helle SjoelundiD, Erin J. CramiD
Abstract
G protein signaling regulates calcium dynamics and contractility in the C. elegans spermatheca. G protein-coupled receptors activate heterotrimeric G proteins, triggering downstream cascades, including the Gs-mediated activation of adenylyl cyclase and subsequent Protein Kinase A (PKA) activation. Our previous work identified GSA-1/Gs and PKA as key modulators of Ca2+ oscillations and tissue contractility within the C. elegans spermatheca. In this study, we show that the inhibitory Gi/o subunit GOA-1 regulates spermathecal transits. We employed TurboID proximity labeling and mass spectrometry to identify 16 candidate interactors of GOA-1. Depletion of these candidates by RNAi did not yield overt spermathecal transit defects.
- organism: C. elegans
- research focus: cell type annotation
- research focus: protein folding & dynamics
- research focus: cell signaling & trafficking
- approach: experimental assay
- Citations
- 2
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