Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited
K. Gwosch, F. Balzarotti, J. K. Pape, P. Hoess, J. Ellenberg, J. Ries, U. Matti, R. Schmidt, S. J. Sahl, S. W. Hell
Abstract
Prakash and Curd provide a re-analysis1 of individual datasets taken from our report2 demonstrating MINFLUX 3D imaging in cells. Their evaluation confirms the unique localization precision provided by MINFLUX2,3 featuring a standard deviation of {sigma} = 1-3 nm. We appreciate their confirmation and also welcome the opportunity to clarify their remaining points. The hitherto almost unconceivable 3D localization precision attained by MINFLUX is likely to hold the key to an all-optical dynamical structural biology.
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