Pathogen lifestyle determines host genetic signature of quantitative disease resistance in oilseed rape (Brassica napus)

O_LICrops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. C_LIO_LIHere, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum. C_LIO_LIWe did not identify any loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen--hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. C_LIO_LIThis is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. C_LIO_LIWe highlight candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. C_LI