Genome biologyCell BiologyPeer-reviewed journal article
An Inflammatory and Quiescent HSC Subpopulation Expands with Age in Humans
Ksenia R. SafinaiD, Basit SalikiD, Dylan KotliariD, Michelle L. CurtisiD, Jonathan GoodiD, Chen WengiD, Shawn David, Soumya RaychaudhuriiD, Antonia KresoiD, Jennifer J. TrowbridgeiD, Vijay G. SankaraniD, Peter van GaleniD
Abstract
Aging of the blood system impacts systemic health and can be traced to hematopoietic stem cells (HSCs). Despite multiple reports on human HSC aging, a unified map detailing their molecular age-related changes is lacking. We developed a consensus map of gene expression in HSCs by integrating seven single-cell datasets. This map revealed previously unappreciated heterogeneity within the HSC population. It also links inflammatory pathway activation (TNF/NF{kappa}B, AP-1) and quiescence within a single gene expression program. This program dominates an inflammatory HSC subpopulation that increases with age, highlighting a potential target for further experimental studies and anti-aging interventions.
- organism: human
- assay: scRNA-seq
- research focus: cell type annotation
- research focus: differential expression
- research focus: gene regulatory network inference
- approach: statistical model
- approach: machine learning (classical)
- Citations
- 3
- FWCI
- 14.87
- Field percentile
- top 2%
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