bioRxivGenomics
HiChIP: Efficient and sensitive analysis of protein-directed genome architecture
Maxwell R Mumbach, Adam J. RubiniD, Ryan A. FlynniD, Chao Dai, Paul A. KhavariiD, William J. GreenleafiD, Howard Y. ChangiD
Abstract
Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.
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