In vivo experimental model of β-glucan-induced innate immune memory

Abstract

SUMMARYInnate immune memory is the ability of innate immune cells to develop a recallable, epigenetically imprinted response after an initial stimulus, enabling them to mount differential responses upon restimulation. Here, we present a detailed protocol of {beta}-glucan preparation, and its in vivo application to modulate innate immune memory in mice through repeated intraperitoneal (IP) injections. This approach results in enhanced hematopoiesis and bone marrow specific myeloid bias, key hallmarks of in vivo innate immune memory responses. For complete details on the use and execution of this protocol, please refer to Hassan et al. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=184 SRC="FIGDIR/small/705736v1_ufig1.gif" ALT="Figure 1"> View larger version (30K): org.highwire.dtl.DTLVardef@d41c3aorg.highwire.dtl.DTLVardef@18939cdorg.highwire.dtl.DTLVardef@31e879org.highwire.dtl.DTLVardef@956f6b_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIPreparation of {beta}-glucan for in vivo experiments. C_LIO_LIIn vivo modulation of innate immune memory through repeated intraperitoneal {beta}-glucan injections in mice. C_LIO_LIModulation of innate immune memory is assessed by enhanced bone marrow hematopoiesis and GMP production. C_LI