Protein Cargo Assessment through Residue Alterations

MotivationThe transport of proteins plays a crucial role in the cellular phenotype. Changes in the protein targeting sequence can result in missing protein delivery to the right destination and at the right time, and can disrupt various cellular pathways. Given the importance of single residue change(s) in the protein targeting sequence we developed a missing computational method. ResultsBy taking into account various protein features like conservation, protein modifications, charge, isoelectric effect and biochemical properties of peptides, the method, TransSite, assess the impact of mutations on the protein transportation. We applied this method to big human (cancer proteins) and nonhuman data, and discovered, in both cases, several phenotype related proteins harbour recurring mutations in their targeting sequences. Availabilityhttps://github.com/AhmedArslan/TransSite Contactaarslan@staford.edu Supplementary informationSupplementary data are available at # online.