eLifeGenomicsPeer-reviewed journal article
Epigenetic Age-Predictor for Mice based on Three CpG Sites
Yang HaniD, Monika Eipel, Julia FranzeniD, Vadim SakkiD, Bertien Dethmers-Ausema, Laura Yndriago, Ander IzetaiD, Gerald de HaaniD, Hartmut GeigeriD, Wolfgang WagneriD
Abstract
Epigenetic clocks for mice were generated based on deep-sequencing analysis of the methylome. In this study we demonstrate that site-specific analysis of DNA methylation levels by pyrosequencing at only three CG dinucleotides (CpGs) in the genes Prima1, Hsf4, and Kcns1 facilitates precise estimation of chronological age in murine blood samples, too. DBA/2J mice revealed accelerated epigenetic aging as compared to C57BL6 mice, which is in line with their shorter life-expectancy. The three-CpG-predictor provides a simple and cost-effective biomarker to determine biological age in large intervention studies with mice.
- organism: mouse
- assay: DNA methylation
- research focus: variant effect prediction
- approach: statistical model
- Citations
- 60
- FWCI
- 2.31
- Field percentile
- top 10%
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