Wolfgang Wagner

This study converts human blood cells into induced neural stem cells, showing prolonged epigenetic de-aging and delayed acquisition of neural stem cell identity over time.

This paper introduces a method using PEG microgels to enable reproducible, scalable iPSC organoid production and differentiation into specific tissues.

This study investigates the relationship between age-related epigenetic changes in DNA methylation, chromatin accessibility, and histone modifications across human aging using integrated WGBS and ATAC-seq data.

This study identifies co-regulated DNA methylation patterns in leukemia and shows their presence in other cancer types, suggesting non-random, network-based epigenetic regulation.

This study uses multimodal single-cell analysis to identify transcription factor networks involved in T-cell aging.

The paper shows that cell loss in RPE cells leads to mechanical changes that impair function, mimicking age-related decline in an in vitro model.

This study shows that the level of myeloid skewing in blood correlates with biological aging and disease in mice and humans.

This study tracks primed-to-naive conversion of pluripotent stem cells using DNA methylation changes and introduces a biomarker for monitoring this process under optimized culture conditions.

This study shows YAP1 promotes cardiomyocyte maturation, sarcomere structure, and contractility in human stem cell-derived cardiomyocytes.

This study shows that protamine overexpression in somatic cells causes chromatin condensation and transcription suppression without altering DNA methylation.

This study shows NSD1 regulates DNA methylation and endodermal differentiation of human iPSCs through HIDEN lncRNA, revealing a novel mechanism linked to Sotos syndrome.

The paper introduces a weighted 2D-kernel density estimation method for epigenetic age prediction, achieving similar accuracy to existing clocks and providing variation scores linked to mortality risk.

This study shows that epigenetic editing at age-associated CpGs can modulate the epigenetic aging landscape, affecting epigenetic clocks and inducing non-random bystander modifications.

The paper identifies aberrant DNA methylation in cancer-associated fibroblasts across multiple cancer types and links these changes to patient prognosis.

This study uses a small molecule inhibitor to rapidly and synchronously induce senescence-like features in human cells, enabling analysis of its core regulatory program without paracrine effects.

CimpleG identifies small CpG methylation signatures for cell type classification and deconvolution, performing efficiently with single CpG sites per cell type.

The paper develops an epigenetic clock for mice using age-associated CpGs conserved between mouse strains and humans, showing high correlation with chronological age.

This study identifies granulocytes as the primary source of exercise-induced cfDNA, which can affect liquid biopsy accuracy in healthy individuals and patients with hematological malignancies.

This study identifies DNA methylation changes during long-term cell culture that resemble epigenetic drift, linked to chromatin conformation changes.

This study compares pyrosequencing, ddPCR, and BBA-seq for epigenetic age prediction in mice, finding that pyrosequencing and ddPCR show slightly better CpG age correlation than BBA-seq.

The paper demonstrates that targeted DNA methylation analysis at individual CpG sites can accurately deconvolve cellular composition in human tissues.

This study develops a new epigenetic age prediction model based on stochastic DNA methylation patterns observed in targeted CpG sites using BBA-seq.

This study tests whether facial aging can serve as a surrogate for biological age in training epigenetic age predictors, finding it provides an independent measure of biological aging.

This study develops a three-CpG site-based epigenetic age predictor for mice using DNA methylation data, showing it can accurately estimate chronological age in blood samples.

The paper shows that human hematopoietic cells maintain normal epigenetic development patterns when transplanted into mice, and epigenetic aging does not accelerate in the xenogeneic environment.

This study shows that specific DNMT3A transcripts have distinct epigenetic and functional effects on hematopoietic differentiation and are linked to AML outcomes.

The paper develops a method to estimate leukocyte counts using DNA methylation analysis, achieving similar precision to conventional methods with advantages in sample handling.

The paper identifies HMGB2 loss as an early event in senescence that disrupts chromatin organization by inducing CTCF clustering across multiple human cell types.

The paper introduces Triplex Domain Finder (TDF), a tool to detect DNA binding domains in lncRNAs by analyzing RNA-DNA interactions, and demonstrates its effectiveness on known triple helix-forming lncRNAs.