Photoperiod stress alters the cellular redox status and is associated with an increased peroxidase and decreased catalase activity

Abstract

Periodic changes of light and dark regulate numerous processes in plants. Recently, a novel type of stress caused by an extended light period has been discovered in Arabidopsis and was named photoperiod stress. Photoperiod stress causes the induction of numerous stress response genes during the night following the extended light period of which many are indicators of oxidative stress. The next day, stress-sensitive genotypes display reduced photosynthetic efficiency and programmed cell death in leaves. Here, we have analysed further the consequences of photoperiod stress and report that it causes changes of the cellular redox status. A prolonged light period caused a strong reduction of the AsA redox during the following night indicating that it induces an oxidizing cellular environment. Further, photoperiod stress was associated with an increased activity of peroxidases and a decreased activity of catalases. Increased peroxidase activity was localized to the apoplast and might be causal for the oxidative stress induced by photoperiod stress.