A slow and clathrin-dependent mode of compensatory endocytosis in hippocampal boutons at near-physiological temperature

Abstract

The mode of presynaptic compensatory endocytosis has been controversial for decades. Recently, an ultrastructural study on synaptic membrane retrieval following synaptic vesicle fusion revealed an ultrafast mechanism of endocytosis in mouse hippocampal synapses at physiological temperature. Using live cell imaging of single-vesicle exo- and endocytosis in mouse hippocampal synapses, combined with accelerated optogenetic acidification of synaptic vesicles, we found that compensatory endocytosis occurs mostly through a slow clathrin-dependent and actin-independent mode at both room and physiological temperatures.