bioRxivGenomicsPreprint
A protocol for high-throughput microplate-based CUT&Tag
Brittany B. Johnson, Gabriel E. BoyleiD, Arnold J FedericoiD, Jing MaiD, Humza HemaniiD, David H. SpenceriD, Jay F. SarthyiD, Michael P. MeersiD
Abstract
Cleavage Under Targets & Tagmentation (CUT&Tag) is a versatile method for measuring genomic occupancy of chromatin-associated proteins with high sensitivity and specificity. CUT&Tag has low sequencing requirements and is therefore suitable for highly multiplexed experiments, but methods to process samples at throughput without specialized equipment are lacking. Here we present a method for simultaneous parallel processing of 96 CUT&Tag samples in a standard microplate. Plate-CUT&Tag can be carried out in a similar time frame to benchtop CUT&Tag and yields data of comparable quality. We present data from cell culture and patient leukemia samples processed with Plate-CUT&Tag to illustrate its utility in large-scale preclinical and translational studies.
- organism: human
- organism: mouse
- assay: ChIP-seq
- research focus: benchmarking
- approach: experimental assay
- Citations
- 0
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