bioRxivBiochemistry
Trefoil factors share a lectin activity that defines their role in mucus
M. A. Jarva, J. P. Lingford, A. John, N. E. Scott, E. D. Goddard-Borger
Abstract
The trefoil factors (TFFs) are disulfide-rich mucosal peptides that protect the epithelium by promoting cell migration and increasing the viscoelasticity of the mucosa. Here we show that all TFFs are divalent lectins that recognise the GlcNAc--1,4-Gal disaccharide, which terminates type-III mucin-like O-glycans. Structural, mutagenic and biophysical data support a model of mucus viscoelasticity that features non-covalent cross-linking of glycoproteins by TFFs.
- assay: proteomics
- research focus: cell signaling & trafficking
- research focus: chromatin & epigenetic regulation
- approach: experimental assay
- approach: biophysical modeling
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