N. E. Scott

The mycobacterial glycoside hydrolase LamH enables capsular arabinomannan release and stimulates growth

This study identifies LamH, a glycoside hydrolase that cleaves -1,6-mannoside linkages in LAM/LM, enabling their export to the capsule and promoting bacterial growth.

Tandem sulfofucolytic-sulfolactate sulfolyase pathway for catabolism of the rare sulfosugar sulfofucose

This paper identifies a novel bacterial pathway for sulfofucose catabolism in Paracoccus wurundjeri, involving a sulfofucolytic Entner-Doudoroff pathway and subsequent biomineralization to sulfite.

FAIMS-enabled N-terminomics analysis reveals novel legumain substrates in murine spleen

This study uses FAIMS to identify novel legumain substrates in murine spleen by improving neo-N-termini detection in proteomic analyses.

Capsular polysaccharide inhibits vaccine-induced O-antigen antibody binding and function across both classical and hypervirulent K2:O1 strains of Klebsiella pneumoniae

This study compares two vaccines targeting K. pneumoniae: one against capsular polysaccharide and one against O-antigen, finding that capsule inhibits O-antigen antibody function and that the capsule vaccine is more effective.

Human gut bacteria tailor extracellular vesicle cargo for the breakdown of diet- and host-derived glycans

This study identifies a mechanism by which Bacteroides thetaiotaomicron tailors its outer membrane vesicle cargo to optimize glycan breakdown, revealing a conserved sorting signal for protein incorporation into vesicles.

Identification of levoglucosan degradation pathways in bacteria and sequence similarity network analysis

The paper identifies two bacteria capable of degrading levoglucosan and analyzes their genome and proteome to understand the degradation pathway and related enzymes.

An expanded proteomic survey of the human parasite Leishmania major focusing on changes in null mutants of the Golgi GDP-Mannose/Fucose/Arabinopyranose transporter LPG2 or the mitochondrial fucosyltransferase FUT1

This paper uses ultradeep proteomics to analyze Leishmania major mutants, revealing significant proteomic changes in LPG2 and FUT1 mutants and highlighting potential therapeutic targets.

InvL, an invasin-like adhesin, is a type II secretion system substrate required for Acinetobacter baumannii uropathogenesis.

This paper identifies InvL, a type II secretion system effector in Acinetobacter baumannii, as an adhesin required for urinary tract infection.

Getting more out of FLAG-Tag co-immunoprecipitation mass spectrometry experiments using FAIMS.

This study shows that using FAIMS in FLAG-Tag co-immunoprecipitation improves protein coverage by reducing tag peptide interference in mass spectrometry.

Burkholderia PglL enzymes are serine preferring oligosaccharidetransferases which target conserved proteins across the Burkholderia genus

Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data

This study evaluates glycoproteomics software performance using human serum datasets, identifies high-performance strategies, and provides recommendations for improved analysis.

The glycoprotease CpaA secreted by medically relevant Acinetobacter species targets multiple O-linked host glycoproteins

This paper identifies CpaA, a glycoprotease from Acinetobacter species, as a broad-spectrum enzyme that cleaves multiple O-linked human glycoproteins, including those involved in complement activation, and proposes its use in glycoproteomics.

A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for diagnosis of glanders infections

This paper constructs a glycoengineered antigen using a conserved Burkholderia O-glycosylation pathway to develop a highly specific diagnostic tool for glanders, achieving 100% specificity and over 88% sensitivity in detecting antibodies from infected horses.

What are we missing by using hydrophilic enrichment? Improving bacterial glycoproteome coverage using total proteome and FAIMS analysis.

This study shows that FAIMS fractionation can enrich glycopeptides missed by HILIC, increasing glycoproteome coverage in bacteria by at least 30%.

Proteomic identification of Coxiella burnetii effector proteins targeted to the host cell mitochondria during infection

This study identifies four novel mitochondrially-targeted effector proteins from Coxiella burnetii using proteomics and organelle purification during native infection.

Open database searching enables the identification and comparison of bacterial glycoproteomes without defining glycan compositions prior to searching

The paper introduces open database searching with wide tolerance to identify and compare bacterial glycoproteomes without prior glycan composition definition, demonstrating its effectiveness across multiple bacterial species.

Trefoil factors share a lectin activity that defines their role in mucus

This study identifies trefoil factors as divalent lectins that bind GlcNAc--1,4-Gal disaccharides, contributing to mucus viscoelasticity through non-covalent glycoprotein cross-linking.

Loss of O-linked protein glycosylation in Burkholderia cenocepacia impairs biofilm formation and siderophore production via alteration of quorum sensing regulation

This paper shows that loss of O-linked glycosylation in B. cenocepacia disrupts quorum sensing, leading to reduced biofilm formation and siderophore production.