Priming of C-glycoside flavones in Colobanthus quitensis with salicylic acid, methyl jasmonate, pimelic acid, suberic acid, and azelaic acid elicits antifungal activity against Botrytis cinerea

Colobanthus quitensis, one of only two native angiosperms in Antarctica, produces C-glycosyl flavones with antifungal activity against Botrytis cinerea. In this study, the exogenous application of the elicitors salicylic acid (SA), methyl jasmonate (MeJA), pimelic acid (PA), suberic acid (SuA), and azelaic acid (AzA) was evaluated for their effect on the accumulation of bioactive metabolites in in vitro-cultivated plants. Exposure to these compounds significantly modulated the expression of key genes in the phenylpropanoid and flavonoid pathways, including pal, chs, chi, fnsII, as well as regulatory genes such as myb12, bhlh, and wrky33, enhancing PAL activity and the accumulation of schaftoside, neoschaftoside, saponarin, and swertiajaponin. This priming process improved the antifungal activity of the extracts, with MeJA and PA identified as the most effective treatments. The in vitro culture approach enabled the assessment of a protected and hard-to-access species without the need for wild harvesting. These results suggest that the exogenous application of elicitors constitutes an efficient strategy to modulate the biosynthesis of specialised metabolites, with implications for the development of biocontrol agents and the improvement of efficiency in sustainable agricultural systems. GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=88 SRC="FIGDIR/small/646165v1_ufig1.gif" ALT="Figure 1"> View larger version (25K): org.highwire.dtl.DTLVardef@67f8corg.highwire.dtl.DTLVardef@9ff3a1org.highwire.dtl.DTLVardef@13403aaorg.highwire.dtl.DTLVardef@1dc95b2_HPS_FORMAT_FIGEXP M_FIG C_FIG