bioRxivBiochemistryPreprint
Antibody-free time-resolved terbium luminescence assays designed for cyclin-dependent kinase 5 (CDK5)
Abstract
Novel time-resolved terbium luminescence assays were developed for CDK5 and CDK2 by designing synthetic substrates which incorporate phospho-inducible terbium sensitizing motifs with kinase substrate consensus sequences. Substrates designed for CDK5 showed no phosphorylation by CDK2, opening the possibility for CDK5-specific assay development for selective drug discovery.
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