Anoop Rama Damodaran

The paper introduces an immunoblot-based activity assay using a near-IR fluorophore-labeled antibody to measure heme-containing histidine kinase activity, overcoming interference from heme fluorescence.

This study enhances the oxygen affinity of the DosS protein by modifying its heme coordination sphere, achieving a 150-fold increase in O2 binding affinity.

The paper demonstrates sequential oxidation of L-lysine by a non-heme iron hydroxylase and computationally analyzes structural elements that enable this reactivity.

This paper uses machine learning and molecular dynamics to design improved variants of a non-heme iron-dependent lysine dioxygenase, achieving higher turnover numbers.

This paper demonstrates how SyrB2 iron halogenase can controllably halogenate non-native substrates to produce mono-, di-, and tri-chlorinated products by adjusting cofactor loading and electron transfer.

This study measures the oxygen binding affinities of DosT and DosS kinases in Mycobacterium tuberculosis, revealing stronger affinities than previously reported and linking them to hypoxia adaptation stages.

The paper describes a method using equilibrium dialysis and HPLC to measure the substrate binding affinity (Kd) of the non-heme iron halogenase BesD for lysine.