bioRxivBioengineering
Lossless Immunocytochemistry using Photo-polymerized Hydrogel Thin-films
J. H. Lee, A. T. Santoso, E. S. Park, K. Matthews, S. P. Duffy, H. Ma
Abstract
Immunocytochemistry (ICC), or immunofluorescence microscopy, is an essential biological technique for phenotyping cells in both research and diagnostic applications. Standard ICC methods often do not work well when the cell sample contains a small number of cells (70% loss) when the sample contains less than 10,000 cells, while encapsulating the cells using a permeable hydrogel thin-film results in a lossless ICC process.
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