C. S. Potter

Early intermediates in bacterial RNA polymerase promoter melting visualized by time-resolved cryo-electron microscopy

This study uses time-resolved cryo-EM to visualize early intermediates in bacterial RNA polymerase promoter melting, revealing key steps in the formation of the transcription-competent open complex.

Pixel Walking along the boreal forest-Arctic tundra ecotone: Large scale ground-truthing of satellite-derived greenness (NDVI)

The paper links satellite-derived vegetation greenness (NDVI) with ground-truth data from the Arctic tundra-boreal forest ecotone, showing how vegetation structure and composition relate to satellite measurements.

Fully Automated Multi-Grid Cryo-EM Screening using Smart Leginon

Smart Leginon automates cryo-EM grid screening, reducing operator time from 6 hours to <10 minutes and improving throughput.

Vitrocam: A simple low cost Vitrobot camera for assessing grid quality

The paper introduces Vitrocam, a low-cost camera to assess cryo-EM grid quality during sample preparation by imaging the Vitrobot process.

System Environmental Metrics Collector for EM facilities

The paper develops a system for monitoring environmental metrics in cryoEM facilities to optimize instrument uptime and data production.

Waffle Method: A general and flexible approach for FIB-milling small and anisotropically oriented samples

The paper introduces the Waffle Method to improve cryo-FIB/SEM workflows for preparing complex biological samples for cryo-ET, demonstrating its effectiveness with various samples and hardware.

Time-resolved cryoEM using Spotiton

The paper introduces a method for preparing cryoEM grids to study short-lived molecular states by rapidly dispensing sample drops and vitrifying them.

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria

The paper presents the cryo-EM structure of AftD from M. abscessus and shows its interaction with ACP, revealing potential regulatory mechanisms.

High Resolution Single Particle Cryo-Electron Microscopy using Beam-Image Shift

This study evaluates the impact of beam-image shift on cryo-EM single-particle reconstruction quality by testing different beam tilt angles and analyzing FSC values and water density peaks.

Complete functional mapping of infection- and vaccine-elicited antibodies against the fusion peptide of HIV

The paper maps functional epitopes of antibodies targeting HIV Env fusion peptide by analyzing mutations affecting neutralization, revealing differences between vaccine- and infection-elicited antibodies.

Routine Determination of Ice Thickness for Cryo-EM Grids

The paper presents two methods to measure ice thickness in cryo-EM grids during data collection to improve sample preparation and data quality.

Routine Single Particle CryoEM Sample and Grid Characterization by Tomography

This study uses fiducial-less tomography to analyze cryoEM grids and finds that 90% of particles are adsorbed to air-water interfaces, impacting protein structure and single particle analysis.