Pseudomonas aeruginosa performs chemotaxis to serotonin, dopamine, epinephrine, and norepinephrine

Abstract

Bacteria use chemotaxis to move to favorable ecological niches. For many pathogenic bacteria, chemotaxis is required for full virulence, particularly for the initiation of host colonization. There do not appear to be limits to the type of compounds that attract bacteria, and we are just beginning to understand how chemotaxis adapts them to their lifestyles. Quantitative capillary assays for chemotaxis show that P. aeruginosa is strongly attracted to serotonin, dopamine, epinephrine, and norepinephrine. Chemotaxis to these compounds is greatly decreased in a mutant lacking the TlpQ chemoreceptor, and complementation of this mutant with a plasmid harboring the tlpQ gene restores wild-type-like chemotaxis. Microcalorimetric titrations of the TlpQ sensor domain with these four compounds indicate that they bind directly to TlpQ. All four compounds are hormones and neurotransmitters that control a variety of processes and are also important signal molecules involved in the virulence of P. aeruginosa. They modulate motility, biofilm formation, the production of virulence factors and serve as siderophores that chelate iron. Therefore, chemotaxis to these four compounds is likely to alter P. aeruginosa virulence. Additionally, we believe that this is the first report of bacterial chemotaxis to serotonin and dopamine. This study provides an incentive for research to define the contribution of chemotaxis to these host signaling molecules to the virulence of P. aeruginosa.