Accurate quantification of canine mitochondrial DNA copy number and its evaluation as a biomarker of brain injury

Abstract

Acute brain injury is challenging to manage in veterinary medicine, with limited validated means of prognostication currently available. The brain is rich in mitochondrial content and contains thousands of copies of mitochondrial DNA (mtDNA) per cell. We hypothesized that brain cell loss following acute brain injury may result in release of mtDNA into the systemic circulation. To investigate this, mtDNA-CN was measured in blood (n=4-6/group) and cerebral cortex (n=1/group) samples from dogs with and without brain injury using absolute quantification by real-time qPCR. By filtering out regions with homology to nuclear mitochondrial insertion sequences (NumtS) and repetitive regions, oligonucleotide primers were designed to the canine mitochondrial and nuclear genomes. In controls, blood mtDNA-CN ranged from 98 to 288 (mean 193{+/-}72), in cases of brain injury, there was a non-significant trend for higher mtDNA-CN, ranging from 163-453 (mean 244{+/-}106). As expected, cerebral cortex contained higher mtDNA-CN than the blood. In a single case with serial sampling, mtDNA-CN increased five days post-injury. We present for the first time an assay to accurately quantify mtDNA-CN in canine samples, with potential as a biomarker for acute brain injury in veterinary practice. Simple SummaryThis work describes a novel assay to accurately measure absolute levels of canine mitochondrial DNA copy number (mtDNA-CN) in biological samples. We describe the range of mtDNA-CN in canine blood and show pilot data suggesting that blood mtDNA-CN should be evaluated as a potential biomarker of acute brain injury.