Telomeric heterochromatin acts in trans to promote meiotic centromere assembly via Aurora B kinase recruitment

During meiosis, chromosomes face a paradox: the machinery that ensures reductional chromosome segregation also destabilizes centromeres by dismantling kinetochores, risking chromosome missegregation. Here we show how cells resolve this crisis through an unexpected activity of the telomere bouquet. We demonstrate that the bouquet transfers heterochromatin components to pericentromeres, which in turn recruit the Aurora B kinase to direct centromere reassembly. The heterochromatin protein Swi6HP1 relocates from telomeres to centromeres, enabling Haspin kinase-dependent phosphorylation of histone H3 and consequent enrichment of the chromosomal passenger complex, which includes the Aurora B kinase. Aurora B then phosphorylates core centromere proteins, including CenpA and CenpC, to promote kinetochore reassembly. Phosphomimetic mutants of CenpA or CenpC bypass the telomere-heterochromatin-Haspin pathway, demonstrating that Aurora B-mediated phosphorylation is sufficient for reassembly. This function is conserved in mitotically proliferating cells subjected to centromere dismantlement. Our findings establish a safeguarded system that couples meiotic nuclear architecture to centromere identity and reveal a fundamental role for the Aurora B kinase in centromere assembly, beyond its canonical function in correcting kinetochore-spindle attachment errors.