Christos Spanos

This study identifies three distinct pathways maintaining kinetochore integrity during meiosis in budding yeast, with distinct temporal requirements and interactions involving Ctf19cCCAN, Mif2CENP-C, and Cse4CENP-A-Okp1CENP-Q-Ame1CENP-U.

This study identifies AKT-like kinase as a regulator of quorum sensing in Trypanosoma brucei, showing its role in differentiation and potential as a therapeutic target.

This study shows that HECT-type ligases UPL3/4 promote the degradation of other E3 ligases like PUB22, thereby regulating immune-related substrate stability and plant immunity.

This study investigates the role of H2A.Z-H2B.V nucleosomes in transcription initiation in Trypanosoma brucei, revealing their unique structural and functional properties.

This study reconstitutes vertebrate meiotic kinetochores to identify conserved factors essential for chromosome segregation in mammalian oocytes.

This study identifies a proteasome function that drives centromeric cohesin removal during meiosis II independently of separase activation, revealing a dual role for the proteasome in chromosome segregation.

This study identifies a conserved mechanism for targeted cohesin loading that organises specialised chromosomal domains in S. pombe.

The paper determines the structure of the apo-PIWIL4-HSP90 complex and shows it adopts an open conformation distinct from piRNA-bound PIWI.

The paper examines how RNA-protein interactions change in memory and cytotoxic T cells under low glucose stress, revealing distinct metabolic and functional responses.

The paper identifies a dysfunction in the SET3-like complex involving TBLR1, ANKRD11, and SETD5 as a molecular basis for neurodevelopmental disorders.

The paper introduces a synthetic SAC strategy to study meiosis II in yeast, revealing differences in kinetochore composition and phosphorylation between meiotic metaphase I and II.

The paper investigates the role of three chromatin-associated PP1 targeting subunits in mitotic exit and G1 re-establishment using multi-omic analyses.

The paper uses synthetic TALE proteins to identify chromatin-associated proteins on repetitive elements in Trypanosoma brucei, revealing their roles in chromosome structure and function.

This study identifies ubiquitin recognition as the mechanism enabling mutual potentiation between cell-surface and intracellular receptors in plant immunity.

This study shows SALL4 binds across the genome to shape chromatin and gene expression in stem cells.

This study identifies PP1 and PP2A phosphatases as key regulators of spindle checkpoint silencing in Cryptococcus neoformans, revealing their recruitment mechanisms and roles in mitotic exit.

This study identifies tetramerisation as essential for SALL transcription factor function, showing its role in DNA binding and linking it to developmental disorders.

The paper shows that yeast rapidly adapts to glucose depletion by using metabolic changes to control translation, prioritizing stress-response genes.

This study reveals how the chromosomal passenger complex (CPC) interacts with nucleosomes to protect centromeric chromatin, showing its non-catalytic role in maintaining chromatin stability.

In DT40 cells, condensins displace extrusive cohesin, bypass cohesive cohesin and stall against each other while extruding ~1-3 kb/sec, yielding irregular helical chromatids of consecutive loops.

The paper identifies Cpn1, a fission yeast ortholog of human Caprin1, as a factor that facilitates heterochromatin establishment by regulating non-coding transcripts and interacting with stress granules.

The paper shows that asymmetric bivalent nucleosomes at developmental gene promoters promote a poised state by recruiting repressive H3K27me3 binders and specific chromatin proteins like KAT6B, which is critical for ESC differentiation.

This study identifies a molecular mechanism by which condensin is recruited to pericentromeres through a conserved interaction between Ycg1 and Sgo1, ensuring proper chromosome segregation.

This study determines the structure and function of KKT23, a kinetochore acetyltransferase in trypanosomes, showing it acetylates histone H2A and interacts with KKT22.

The paper identifies PLK1-mediated phosphorylation of Mis18 and Mis18BP1 as essential for activating the Mis18 complex to ensure CENP-A loading and centromere inheritance.

This study improves RNA-interactome detection using DIA SILAC over DDA SILAC, achieving higher protein detection and lower variation with single-shot analysis.

The paper identifies DAXX as a novel histone chaperone pathway that deposits H3.3K9me3 before DNA deposition, expanding the histone chaperone network's role in heterochromatin assembly.

The paper identifies the SPARC complex in Trypanosoma brucei, showing it defines RNAPII promoters and regulates transcription start site positioning and directionality.

The paper introduces RP-CONA, a method to identify RNA-protein interaction modulators, and finds quercetin inhibits HuR-pri-miR-7-1 interaction, increasing miR-7 and reducing α-Synuclein.

The paper identifies three TRAMP complexes in yeast and shows Trf4/Trf5 confer substrate specificity, with TRAMP binding co-enriching functionally related mRNAs.