Formation of Condition-Dependent Alpha-Synuclein Fibril Strain in Artificial Cerebrospinal Fluid

-Synuclein (aSyn) is an intrinsically disordered protein involved in neurotransmission and synaptic plasticity. The pathological aggregation of this protein is a hallmark of synucleinopathies such as Parkinsons disease (PD) or Multiple System Atrophy (MSA). Misfolded aSyn, which primarily originates in cell cytosol, transmits between neurons, promoting a prion-like propagation. However, the extracellular environments such as interstitial and cerebrospinal fluids (ISF & CSF) play a major role in its clearance and pathological transformation. The molecular components of CSF, including proteins, glycosaminoglycans, and metal ions may influence the aggregate morphology, structure and cytotoxicity to cells. To better understand how extracellular composition affects aggregates and their formation, we employed artificial cerebrospinal fluid (aCSF) to mimic potential aggregation processes occurring in CSF. We observed distinct aCSF-specific aSyn fibrils that exhibited low stability outside aCSF, and the removal of key CSF components led to its structural alterations. Cryo-electron microscopy revealed that these fibrils possess an electron density pocket coordinated with polar basic AAs (K43, K45, H50) that is also observed in aggregates obtained from MSA patients. Our findings illustrate the importance of physiologically relevant conditions in studying aSyn aggregation and may explain why disease-related fibril structure replication in vitro has not yet been successful.