All about urine: Longitudinal examination of urine pH, specific gravity, proteins, culture, and resistance profiles in healthy dogs

BackgroundUrine is routinely evaluated in dogs to assess health. Reference ranges for many urine properties are well established, but the scope of variation in these properties over time within heathy dogs is not well characterized. ObjectivesLongitudinally characterize urine properties in healthy dogs over 3 months. AnimalsFourteen healthy client-owned dogs. MethodsDogs were evaluated for health; then, mid-stream free catch urine was collected from each dog at 12 timepoints: Mornings / afternoons of Days 1, 2, 3; end of Weeks 4, 5, 6, 7 and Months 2 and 3. Urine pH, urine specific gravity (USG), protein, cultures, and antimicrobial resistance profiles were evaluated at each timepoint. ResultsUrine pH varied significantly within and between dogs over time (Friedmans test: within p = 0.031; between p 105 CFU/mL) and should not be considered the only indicator of urinary tract infection. 3) Bacterial isolates demonstrated widespread resistance to amoxicillin / oxacillin underscoring the need for antimicrobial stewardship.