Widespread Corticothalamic Connectivity Identifies the Inferior Pulvinar as a Central Node in Visual Network Architecture

The medial subdivision of the inferior pulvinar (PIm) has been implicated in motion processing, visuomotor integration, and residual visual function, yet a comprehensive account of its cortical inputs remains unresolved. Previous studies often relied on indirect cortical injections or tracer deposits spanning multiple pulvinar subdivisions, limiting anatomical specificity. Here, we used MRI-guided, cytoarchitectonically restricted retrograde tracer injections to selectively target PI in the common marmoset (Callithrix jacchus) and systematically map its cortical afferents. Across four cases, retrogradely labeled neurons were widely distributed throughout occipital, temporal, parietal, and cingulate cortices, with a strong predominance in layer V, consistent with driver-like corticothalamic projections. Early and middle-tier visual areas (V1, V2, V3, V3A, V4, V6/DM) contributed substantial input, with labeling patterns corresponding to peripheral visual field representations. The middle temporal complex (MT, MTc, MST, FST) represented one of the densest sources of cortical projections. Prominent inputs also arose from posterior parietal regions, including LIP, MIP, VIP, AIP, and inferior parietal areas (e.g., PFG, OPt), linking PIm to visuospatial and action-related networks. Semi-quantitative analyses indicated that occipital cortex and the MT complex together accounted for approximately 60% of total cortical input, while parietal cortex contributed roughly 20%. Additional projections from retrosplenial and posterior cingulate cortices were observed. These findings identify PIm as a central integrative node embedded within distributed visual and visuomotor networks. Rather than functioning as a restricted visual relay, PIm appears positioned to coordinate motion, spatial, and action-relevant signals within cortico-thalamocortical circuits supporting adaptive visually-guided behavior.