Mouse pups lacking a cerebral cortex develop abnormal vocal behavior

Abstract

Vocal learning is an essential component of spoken language and critically depends on the cerebral cortex. The evolutionary origins of cortical/pallial control over vocal learning abilities in mammals and songbirds remains largely unknown. For instance, reports conflict on whether the cerebral cortex contributes, in any way, to vocal communication in vocal non-learning mice. Physiological studies in adult mice have shown that regions of the motor cortex have roles in modulation of vocalizations in mice, yet, genetic ablation of the cerebral cortex reportedly has minimal, if any, impact on mouse vocal behavior. Re-analysis of adult acortical mice revealed that deep learning machine classifiers could distinguish mutant ultrasonic vocalizations from wildtypes. However, the specific acoustic features underlying these differences were not identified. Here, we investigated isolation calls of acortical mouse pups using statistical analysis of acoustic features and playback experiments to determine whether mutants lacking a cerebral cortex have altered vocal development. We find that a subset of acoustic features differ between acortical and wildtype pup vocalizations and that these differences are indicative of distress. Moreover, call bouts of acortical pups have lower informational complexity that are more comparable to random probability sampling. Playbacks indicate that dams preferentially approach vocalizations of acortical pups. Our analyses provide evidence that the murine cerebral cortex influences development of complex vocal behaviors, suggesting mice can be used to gain useful insights into the foundations of vocal learning.