bioRxivGenomicsPreprint
HiVA: an integrative wet- and dry-lab platform for haplotype and copy number analysis of single-cell genomes
Masoud Zamani EstekiiD, Amin ArdeshirdavaniiD, Daniel AlcaideiD, Heleen MassetiD, Jia DingiD, Alejandro SifrimiD, Parveen KumariD, Eftychia DimitriadouiD, J. AertsiD, Thierry VoetiD, Yves MoreauiD, Joris Robert VermeeschiD
Abstract
Haplotyping is imperative for comprehensive analysis of genomes, imputation of genetic variants and interpretation of error-prone single-cell genomic data. Here we present a novel sequencing-based approach for whole-genome SNP typing of single cells, and determine genome-wide haplotypes, the copy number of those haplotypes as well as the parental and segregational origin of chromosomal aberrations from sequencing- and array-based SNP landscapes of single cells. The analytical workflow is made available as an interactive web application HiVA (https://hiva.esat.kuleuven.be).
- assay: whole-genome sequencing
- research focus: variant calling
- research focus: software / tool
- approach: experimental assay
- approach: statistical model
- Citations
- 5
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