MATRIEX Imaging: Multi-Area Two-photon Real-time In-vivo Explorer

Mengke Yang; Zhenqiao Zhou; Jianxiong Zhang; Shanshan Jia; Tong Li; Jiangheng Guan; Xiang Liao; Bing Leng; Jing Lyu; Kuan Zhang; Min Li; Yan Jun Gong; Zhiming Zhu; Junan Yan; Yi Zhou; Jian K. Liu; Zsuzsanna Varga; Arthur Konnerth; Yuguo Tang; Jinsong Gao; Xiaowei Chen; Hongbo Jia

doi:10.1038/s41377-019-0219-x

NeuroscienceLight Science & ApplicationsNov 28, 2019

Peer-reviewed journal article

MATRIEX Imaging: Multi-Area Two-photon Real-time In-vivo Explorer

Mengke Yang, Zhenqiao Zhou iD, Jianxiong Zhang iD, Shanshan Jia iD, Tong Li iD, Jiangheng Guan, Xiang Liao iD, Bing Leng, Jing Lyu iD, Kuan Zhang iD, Min Li iD, Yan Jun Gong iD, Zhiming Zhu iD, Junan Yan iD, Yi Zhou iD, Jian K. Liu iD, Zsuzsanna Varga, Arthur Konnerth iD, Yuguo Tang iD, Jinsong Gao, Xiaowei Chen iD, Hongbo Jia iD

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Abstract

Two-photon laser scanning microscopy, originally developed since 1990s1, has been widely applied for biomedical research in recent decades, particularly popular among neuroscientists for studying neural functions in vivo2. However, it is typically restricted to one imaging area that is orthogonal to the optical axis. Here, we demonstrate a novel multi-axis optical conjugation method that enables two-photon imaging at single-cell resolution simultaneously in multiple areas at different depths, each of which could have a view diameter of ~200 m and could be largely freely targeted within a zone up to 12-mm diameter. For example, we show simultaneous imaging of neuronal activities in the primary visual cortex (V1), the primary motor cortex (M1) and the hippocampal CA1 region of awake mice. This method can be readily implemented on a single conventional two-photon microscope to enable multi-area exploration of neuronal activities in vivo.