The paper develops two variants of a glutamate indicator with enhanced sensitivity and tailored deactivation rates for studying synaptic transmission in vivo.

Nature Methods · Dec 23, 2025Peer-reviewedNeuroscience

Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

Abhi Aggarwal, Rui Liu, Yang Chen, Amelia J. Ralowicz, Samuel J Bergerson, Filip Tomáška, Boaz Mohar, Timothy Hanson, Jeremy P. Hasseman, Daniel Reep, Getahun Tsegaye, Pantong Yao, Xiang Ji, Marinus Kloos, Deepika Walpita, , Kaspar Podgorski

This paper develops improved glutamate indicators with faster activation kinetics and better synaptic localization for imaging synaptic transmission in neurons.

Nature Methods · May 4, 2023Peer-reviewedNeuroscience

MATRIEX Imaging: Multi-Area Two-photon Real-time In-vivo Explorer

Mengke Yang, Zhenqiao Zhou, Jianxiong Zhang, Shanshan Jia, Tong Li, Jiangheng Guan, Xiang Liao, Bing Leng, Jing Lyu, Kuan Zhang, Min Li, Yan Jun Gong, Zhiming Zhu, Junan Yan, Yi Zhou, , Hongbo Jia

The paper introduces a method for multi-area two-photon imaging in awake mice, enabling simultaneous single-cell resolution imaging across different brain regions.

Light Science & Applications · Nov 28, 2019Peer-reviewedNeuroscience

High-performance GFP-based calcium indicators for imaging activity in neuronal populations and microcompartments

Hod Dana, Yi Sun, Boaz Mohar, Brad K. Hulse, Jeremy P. Hasseman, Getahun Tsegaye, Arthur Tsang, Allan M. Wong, Ronak H. Patel, John J Macklin, Yang Chen, Arthur Konnerth, Vivek Jayaraman, Loren L. Looger, Eric R. Schreiter, , Douglas S. Kim

Structure-guided mutagenesis of GCaMP6 plus neuron-based screening yields a jGCaMP7 family, each variant tuned to a different regime: single spikes, neurites and neuropil, or population imaging.

bioRxiv · Oct 3, 2018 · PreprintNeuroscience