Nucleoid associated proteins and their effect on E. coli chromosome

Abstract

A seemingly random and disorganized bacterial chromosome, in reality, is a well organized nucleus-like structure, called the nucleoid, which is maintained by several nucleoid associated proteins(NAPs). Here we present an application of a previously developed Hi-C based computational method to study the effects of some of these proteins on the E. coli chromosome. Simulations with encoded Hi-C data for mutant, hupAB deficient, E. coli cells, revealed a decondensed, axially expanded chromosome with enhanced short range and diminished long range interactions. Simulations for mutant cells deficient in FIS protein revealed that the effects are similar to that of the hupAB mutant, but the absence of FIS led to a greater disruption in chromosome organization. Absence of another NAP, MatP, known to mediate Ter macrodomain isolation, led to enhanced contacts between Ter and its flanking macrodomains but lacked any change in matS sites localization. Deficiency of MukBEF, the only SMC complex present in E. coli, led to disorganization of macrodomains. Upon further analysis, it was observed that the above mutations do not significantly impact the local chromosome organization (~ 100 Kb) but only affect the chromosome on a larger scale (>100 Kb). These observations shed more light on the sparsely explored effects of NAPs on the overall chromosome organization and helps us understand the myriad complex interactions NAPs have with the chromosome.